期刊
FRONTIERS IN ECOLOGY AND EVOLUTION
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fevo.2022.909865
关键词
methanotrophs; methanogens; methane; karst caves; biogeochemical cycles
类别
Karst caves may serve as a sink for atmospheric methane, with Gammaproteobacterial MOB being the most abundant microbial community and Methanosarcina dominating the methanogens. Additionally, we have created a cave ecosystems protein database (CEPD) for gene profiling.
Karst ecosystems represent up to 25% of the land surface and recent studies highlight their potential role as a sink for atmospheric methane. Despite this, there is limited knowledge of the diversity and distribution of methane-oxidizing bacteria (MOB) or methanogens in karst caves and the sub-surface environment in general. Here, we performed a survey of 14 shotgun metagenomes from cave ecosystems covering a broad set of environmental conditions, to compare the relative abundance and phylogenetic diversity of MOB and methanogens, targeting biomarker genes for methane monooxygenase (pmoA and mmoX) and methyl-coenzyme M reductase (mcrA). Taxonomic analysis of metagenomes showed 0.02-1.28% of classified reads were related to known MOB, of which Gammaproteobacterial MOB were the most abundant making up on average 70% of the surveyed caves' MOB community. Potential for biogenic methane production in caves was also observed, with 0.008-0.39% of reads classified to methanogens and was dominated by sequences related to Methanosarcina. We have also generated a cave ecosystems protein database (CEPD) based on protein level assembly of cave metagenomes that can be used to profile genes of interest.
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