4.5 Article

Morphological and molecular approaches for identification of murine Eimeria papillata infection

期刊

出版社

ELSEVIER
DOI: 10.1016/j.jksus.2022.102164

关键词

Apicomplexa; Eimeria papillata; Rodents; Morphology; PCR; 18SrDNA; ITS1

资金

  1. Princess Nourah bint Abdulrahman University Researchers Supporting Project [PNURSP2022R23]
  2. Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia [RSP-2021/25]
  3. King Saud University, Riyadh, Saudi Arabia

向作者/读者索取更多资源

This study morphologically described and molecularly characterized oocysts recovered from Mus musculus, identifying them as Eimeria papillata. The ITS1 region was found to be a potentially useful marker for differentiating strains of Eimeria species in rodents.
Oocysts recovered from Mus musculus were morphologically described and molecularly characterized using 18S rDNA and ITS1 regions. Sporulated oocysts had two layers and were sub-spherical measuring 20.76 (19.41-22.33) x 17.46 (15.44-19.15) lm with a length/width index of 1.18. Sporocysts were with stieda and substieda bodies with sporocystic residuum. Molecular data from both 18S rDNA and ITS1 regions revealed that the species under investigation is related to Eimeria papillata. The 18S rDNA sequences obtained in this investigation were identical to an E. papillata sequence from M. musculus found in GenBank. The sequence obtained from the ITS-1 region showed a slight difference from other sequences from E. papillata for the same region with a similarity percent of 97.2% to 100%. The AT content of the ITS1 region from the present study was found to be 53.1%. According to ITS1 data, 10 haplotypes were characterized with haplotype diversity (Hd) of 0.9271. Sequences of the ITS1 region from E. papillata were variable in 18 sites with 3 indels, of those variable sites 15 were transitions while 3 were transversions. Therefore; the ITS1 region is probably a good marker for differentiating different strains of Eimeria species in rodents. (c) 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据