期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 181, 期 -, 页码 221-228出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2016.01.040
关键词
Anxiety; Central nervous system; Coumarins; Ethnobotany; Sedative; Tagetes lucida
资金
- CONACYT [226454, INP-NC123280]
- Posgrado en Ciencias Biologicas of UNAM [CONACYT-332465]
Ethnopharmacological relevance: Morelos State is one of the most important regions of Mexico where several plant species are used in traditional medicine to influence central nervous system (CNS) activity; for example Tagetes lucida Cav. Aim of the study: To investigate the ethnobotany, phytochemistry and pharmacology of the tranquilizing properties of T. lucida aerial parts. Material and methods: Data on the medicinal uses of T. lucida were explored by interviewing healers and merchants of local markets in different regions of Morelos State by using a questionnaire. Anxiolytic and/or sedative-like responses of the T. lucida were investigated in experimental models in mice such as: open-field, exploration cylinder, hole-board, plus-maze, and the barbituric-induced hypnosis potentiation. The possible mechanism of action was explored in the presence of WAY100635 (0.32 mg/kg, i.p.) and flumazenil (10 mg/kg, i.p.) antagonists. A feasible active compound was isolated and identified by using conventional chromatography, including UHPLC and MS (DART) [M+H]+ techniques. Results: Interviews of healers and merchants from ten local regions of Morelos State showed that they recommended T. lucida as infusion and as tincture for several culture-bound syndromes associated with the CNS. Anxiolytic and sedative-like activities of polar extracts were corroborated in the experimental models; these effects were inhibited in the presence of 5-HT1A and GABA/BDZ receptor antagonists. Dimethylfraxetin was identified as one possible active compound. Conclusions: The results support the anxiolytic and sedative-like properties of T. lucida in traditional medicine by involving serotonergic and GABAergic neurotransmission and coumarinic constituents. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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