期刊
ANTIBIOTICS-BASEL
卷 11, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/antibiotics11060787
关键词
dermaseptin; DRS-B2; alginate nanoparticles; menthol; lactic acid; synergy; potentialization; cytotoxicity
资金
- ANR [Sincolistin-15-CE21-0015]
- ALIBIOTECH from la Region des Hauts de-France
- CPER Photonics for Society
- CNRS, University of Lille
Dermaseptin B2 (DRS-B2) is an antimicrobial peptide secreted by the Amazonian tree frog, Phyllomedusa bicolor. This study demonstrates that adsorption of DRS-B2 onto alginate nanoparticles enhances its antibacterial activity, particularly against colistin-resistant strains of Escherichia coli. The addition of lactic acid or menthol further enhances the antibacterial activity of this new formulation. Safety and stability tests conducted on human erythrocytes and eukaryotic cell lines indicate the potential of these formulations as alternatives to antibiotics for treating Gram-negative bacterial infections such as E. coli.
Dermaseptin B2 (DRS-B2) is an antimicrobial peptide secreted by Phyllomedusa bicolor, which is an Amazonian tree frog. Here, we show that the adsorption of DRS-B2 on alginate nanoparticles (Alg NPs) results in a formulation (Alg NPs + DRS-B2) with a remarkable antibacterial activity against Escherichia coli ATCC 8739 and E. coli 184 strains, which are sensitive and resistant, respectively, to colistin. The antibacterial activity, obtained with this new formulation, is higher than that obtained with DRS-B2 alone. Of note, the addition of lactic acid or menthol to this new formulation augments its antibacterial activity against the aforementioned Gram-negative bacilli. The safety of DRS-B2, and also that of the new formulation supplemented or not with a small molecule such as lactic acid or menthol has been proven on the human erythrocytes and the eukaryotic cell line types HT29 (human) and IPEC-1 (animal). Similarly, their stability was determined under the conditions mimicking the gastrointestinal tract with different conditions: pH, temperature, and the presence of digestive enzymes. Based on all the obtained data, we assume that these new formulations are promising and could be suggested, after in vivo approval and completing regulation aspects, as alternatives to antibiotics to fight infections caused by Gram-negative bacilli such as E. coli.
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