期刊
FOODS
卷 11, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/foods11131930
关键词
high hydrostatic pressure; ionic liquids; aqueous biphasic systems; Momordica charantia L.; saponins
资金
- National Key R&D Program of China [2018YFC1602201]
- Key Program for Shaanxi Science and Technology [2020NY-146]
- Lueyang Black-Bone Chicken Industry Development Research Institute [WJYJY-2021-9]
This study successfully extracted the total saponins from Momordica charantia using high hydrostatic pressure (HHP) technology, and purified them using an ionic liquid-based aqueous biphasic system. The purity of the saponins was significantly improved, providing a basis for further research on their biological activity.
Momordica charantia L. (Cucurbitaceae) is rich in saponins, which have multiple biological effects. In this study, the total saponins of M. charantia were extracted by high hydrostatic pressure (HHP) technology. The optimal extraction process was determined (ethanol concentration 68%, pressure-holding time 8 min, ratio of material to solvent 1:35 and pressure 510 MPa), and the extraction amount of saponins reached 127.890 mg/g. On this basis, an ionic liquid-based aqueous biphasic system was constructed to purify the total saponins. Under the optimized conditions, the purity of M. charantia saponins was 76.06%. Liquid chromatography-mass spectrometry (LC/MS) was used to characterize the saponins in the purified extract of M. charantia. It was found that there were four kinds of saponins in the extract of M. charantia: kuguaglycoside A, momordicoside L, kuguacin B and kuguacin J, providing a basis for the study of the biological activity of saponins.
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