期刊
FOODS
卷 11, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/foods11152351
关键词
chicken; meat adulteration; DNA extraction; polymerase chain reaction; lateral flow strip
资金
- National Natural Science Foundation of China [32102060]
- Key R&D Program of Zhejiang [2021C02059, 2022C02028]
- Natural Science Foundation of Zhejiang Province [LQ21B050003]
- Science and Technology Programs of Ningbo [202003N4127, 20211ZDYF020179]
- State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts [2021DG700024-KF202112]
- Science and Technology Innovation Leading Talent Project of Zhejiang [2021R52046]
The aim of this study was to develop an accurate, easy-to-use, and cost-effective method for detecting chicken adulteration using PCR and LFS. The study compared six DNA extraction methods and identified a suitable one. The PCR-LFS method demonstrated high specificity and sensitivity, providing accurate results in a short time.
The aim of this study was to develop an accurate, easy-to-use, and cost-effective method for the detection of chicken adulteration based on polymerase chain reaction (PCR) and lateral flow strip (LFS). We compared six DNA extraction methods, namely the cetyltrimethylammonium bromide (CTAB) method, salt method, urea method, SDS method, guanidine isothiocyanate method, and commercial kit method. The chicken cytb gene was used as a target to design specific primers. The specificity and sensitivity of the PCR-LFS system were tested using a self-assembled lateral flow measurement sensor. The results showed that the DNA concentration obtained by salt methods is up to 533 +/- 84 ng mu L-1, is a suitable replacement for commercial kits. The PCR-LFS method exhibits high specificity at an annealing temperature of 62 degrees C and does not cross-react with other animal sources. This strategy is also highly sensitive, being able to detect 0.1% of chicken in artificial adulterated meat. The results of the test strips can be observed with the naked eye within 5 min, and this result is consistent with the electrophoresis result, demonstrating its high accuracy. Moreover, the detection system has already been successfully used to detect chicken in commercial samples. Hence, this PCR-LFS strategy provides a potential tool to verify the authenticity of chicken.
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