期刊
FOODS
卷 11, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/foods11131925
关键词
immunomodulation; polysaccharide; fermented Morinda citrifolia L.; RAW 264.7; Balb/c mice
资金
- Technology development Program - Ministry of SMEs and Startups (MSS, Korea) [S2840161]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education (MOE, Korea) [NRF-2021R1A6A1A03044242]
- Fostering Outstanding Universities for Research through the National Research Foundation of Korea (NRF) - Ministry of Education (MOE, Korea) [BK21 FOUR-4299990913942]
- Korea Technology & Information Promotion Agency for SMEs (TIPA) [S2840161] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study found that polysaccharide fraction from fermented M. citrifolia L. has immunomodulatory effects, increasing NO production and proinflammatory cytokine release in macrophages. Administration of the polysaccharide to mice also increased immune cell numbers and cytokine production, as well as enhancing NK cytotoxicity in the abdomen and spleen.
This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW 264.7 cells exposed to FMP (50, 100, and 200 mu g/mL) for 24 h showed increased NO production, proinflammatory cytokine (IL-1 beta, IL-6, and TNF-alpha) release, and COX-2 and iNOS protein expression. FMP (100, 200 mg/kg) and deacetylasperulosidic acid (DAA) (20 mg/kg) administered orally to Balb/c mice for 14 days upregulated NO production and NK cytotoxicity in abdominal cavity and spleen, respectively. Th1 and Th2 cytokines production and immune cell numbers increased in spleen, mesenteric lymph nodes (MLN), peritoneal exudate cells (PEC), Peyer's patches (PP), and peripheral blood mononuclear cells (PBMC). Therefore, FMP containing DAA can be used as materials for health functional foods to enhance immune responses.
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