期刊
FRONTIERS IN MARINE SCIENCE
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmars.2022.939716
关键词
omega-3 polyunsaturated fatty acid; docosahexaenoic acid (DHA); PUFA beta-oxidation; 2; 4-dienoyl-CoA reductase (DECR); Aurantiochytrium sp
Disrupting the fatty acid beta-oxidation pathway through metabolic engineering in Aurantiochytrium sp. enables the construction of a high-yielding DHA production strain. Knockout of the 2,4-dienyl-CoA reductase gene resulted in increased neutral lipids and DHA production.
Docosahexaenoic acid (DHA) has attracted attention from researchers because of its pharmacological and nutritional importance. Currently, DHA production costs are high due to fermentation inefficiency; however, improving DHA yield by metabolic engineering in thraustochytrids is one approach to reduce these costs. In this study, a high-yielding (53.97% of total fatty acids) DHA production strain was constructed by disrupting polyunsaturated fatty acid beta-oxidation via knockout of the 2,4-dienyl-CoA reductase (DECR) gene (KO strain) in Aurantiochytrium sp. Slight differences in cell growth was observed in the wild-type and transformants (OE and KO), with cell concentrations in stationary of 2.65x10(6), 2.36x10(6) and 2.56x10(6) cells mL(-1) respectively. Impressively, the KO strain yielded 21.62% more neutral lipids and 57.34% greater DHA production; moreover, the opposite was observed when overexpressing DECR (OE strain), with significant decreases of 30.49% and 64.61%, respectively. Furthermore, the KO strain showed a prolonged DHA production period with a sustainable increase from 63 to 90 h (170.03 to 203.27 mg g(-1) DCW), while that of the wildtype strain decreased significantly from 150.58 to 140.10 mg g(-1) DCW. This new approach provides an advanced proxy for the construction of sustainable DHA production strains for industrial purposes and deepens our understanding of the metabolic pathways of Aurantiochytrium sp.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据