期刊
METABOLITES
卷 12, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/metabo12070583
关键词
bile acids; ring trial; LC-MS/MS; absolute quantification; human serum; murine serum
资金
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [403224013-SFB 1382, 395357507-SFB 1371, TRR333/1, AOBJ: 450149205, AOBJ: 624808, AOBJ: 667675, AOBJ: 676866]
- German Federal Ministry of Education and Research (BMBF), Germany [FKZ: 01EO1501]
- Else Kroener Fresenius Foundation
- IFB Adiposity Disease
This study compares and harmonizes methodologies for quantification of bile acids, including primary and secondary bile acids, in different samples. The results show that measurement of hydrophobic bile acids is the most challenging, but the quality assessments are generally similar, and data from chosen locations can be used for comparisons between studies.
Bile acids are a key mediator of the molecular microbiome-host interaction, and various mass spectrometry-based assays have been developed in the recent decade to quantify a wide range of bile acids. We compare existing methodologies to harmonize them. Methodology for absolute quantification of bile acids from six laboratories in Europe were compared for the quantification of the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) and conjugated products glycocholic acid (GCA) and taurocholic acid (TCA). For the bacterially modified secondary bile acids, the quantification of deoxycholic acid (DCA) and lithocholic acid (LCA) was compared. For the murine bile acids, we used the primary muricholic acids (alpha-MCA and, beta-MCA) and the intestinally produced secondary bile acid muricholic (omega-MCA). The standards were spiked into methanol:water (1:1) mix as well as in human and murine serum at either low concentration range (150-3000 nM) or high concentration range (1500-40,000 nM). The precision was better for higher concentrations. Measurements for the hydrophobic unconjugated bile acids LCA and omega-MCA were the most challenging. The quality assessments were generally very similar, and the comprehensive analyses demonstrated that data from chosen locations can be used for comparisons between studies.
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