4.6 Article

Cell Cycle Progression Influences Biofilm Formation in Saccharomyces cerevisiae 1308

期刊

MICROBIOLOGY SPECTRUM
卷 10, 期 3, 页码 -

出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/spectrum.02765-21

关键词

cell cycle; biofilm; S; cerevisiae; CLN3; SIC1; ACE2

资金

  1. National Key R&D Program of China [2021YFC2101100, 2018YFB1501700]
  2. National Key Research and Development Program of China [2018YFB1501705]
  3. Key Program of the National Natural Science Foundation of China [21636003]
  4. Outstanding Youth Foundation of China [SBK2017010373]
  5. Program for Changjiang Scholars and Innovative Research Team in University [IRT_14R28]
  6. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)
  7. Technology Support Program of Jiangsu [BE2014715]
  8. Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
  9. Key Research and Development Program of Nanjing Jiangbei New Area [ZDYF20200220]

向作者/读者索取更多资源

Immobilised fermentation using biofilm resistance can improve fermentation efficiency and yield, and the cell cycle influences biofilm formation by regulating cell adhesion and polysaccharide formation.
Immobilised fermentation can be achieved using biofilm resistance, resulting in improved fermentation efficiency and yield. The link between the cell cycle and biofilms deserves further study since reports are lacking in this area. Biofilm-immobilized continuous fermentation is a novel fermentation strategy that has been utilized in ethanol fermentation. Continuous fermentation contributes to the self-proliferation of Saccharomyces cerevisiae biofilms. Previously, we successfully described the cell cycle differences between biofilm-immobilized fermentation and calcium alginate-immobilized fermentation. In the present study, we investigated the relationship between biofilm formation and the cell cycle. We knocked down CLN3, SIC1, and ACE2 and found that Delta cln3 and Delta sic1 exhibited a predominance of G(2)/M phase cells, increased biofilm formation, and significantly increased extracellular polysaccharide formation and expression of genes in the FLO gene family during immobilisation fermentation. Delta ace2 exhibited a contrasting performance. These findings suggest that the increase in the proportion of cells in the G(2)/M phase of the cell cycle facilitates biofilm formation and that the cell cycle influences biofilm formation by regulating cell adhesion and polysaccharide formation. This opens new avenues for basic research and may also help to provide new ideas for biofilm prevention and optimization. IMPORTANCE Immobilised fermentation can be achieved using biofilm resistance, resulting in improved fermentation efficiency and yield. The link between the cell cycle and biofilms deserves further study since reports are lacking in this area. This study showed that the ability of Saccharomyces cerevisiae to produce biofilm differed when cell cycle progression was altered. Further studies suggested that cell cycle regulatory genes influenced biofilm formation by regulating cell adhesion and polysaccharide formation. Findings related to cell cycle regulation of biofilm formation set the stage for biofilm in Saccharomyces cerevisiae and provide a theoretical basis for the development of a new method to improve biofilm-based industrial fermentation.

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