期刊
ANTIOXIDANTS
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/antiox11081533
关键词
arabidopsis; chloroplast; inducible expression; iron-sulfur; NEET; proteomics; ROS; thioredoxin
资金
- National Science Foundation [IOS-2110017, IOS-1353886, MCB-1936590, IOS-1932639, MCB-2224839]
- Bond Life Sciences Early Concept Grant
- Interdisciplinary Plant Group
- University of Missouri
NEET proteins are conserved 2Fe-2S proteins that regulate iron and reactive oxygen species levels in plant and mammalian cells. Disrupting AtNEET function causes oxidative stress, chloroplast iron overload, activation of iron-deficiency responses, and cell death. This study reveals new roles for AtNEET in supporting the ferredoxin-thioredoxin network, iron mobilization, and cytosolic 2Fe-2S cluster assembly in plants.
NEET proteins are conserved 2Fe-2S proteins that regulate the levels of iron and reactive oxygen species in plant and mammalian cells. Previous studies of seedlings with constitutive expression of AtNEET, or its dominant-negative variant H89C (impaired in 2Fe-2S cluster transfer), revealed that disrupting AtNEET function causes oxidative stress, chloroplast iron overload, activation of iron-deficiency responses, and cell death. Because disrupting AtNEET function is deleterious to plants, we developed an inducible expression system to study AtNEET function in mature plants using a time-course proteomics approach. Here, we report that the suppression of AtNEET cluster transfer function results in drastic changes in the expression of different members of the ferredoxin (Fd), Fd-thioredoxin (TRX) reductase (FTR), and TRX network of Arabidopsis, as well as in cytosolic cluster assembly proteins. In addition, the expression of Yellow Stripe-Like 6 (YSL6), involved in iron export from chloroplasts was elevated. Taken together, our findings reveal new roles for AtNEET in supporting the Fd-TFR-TRX network of plants, iron mobilization from the chloroplast, and cytosolic 2Fe-2S cluster assembly. In addition, we show that the AtNEET function is linked to the expression of glutathione peroxidases (GPXs), which play a key role in the regulation of ferroptosis and redox balance in different organisms.
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