期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.942077
关键词
exosomes; extracellular vesicles; exosome isolation; magnetic bead; ultracentrifugation
资金
- Zhejiang Provincial Natural Science Foundation of China
- [LGF21H200007]
In this study, commonly used exosome isolation approaches are reviewed, with a focus on magnetic bead-based capture, which offers high purity and integrity of exosomes. The challenges of traditional methods for exosome isolation are also described, highlighting the directions for future research and development.
Exosomes, one type of extracellular vesicle (EV) secreted by cells, participate in intercellular communication and other biological processes as carriers of lipids, functional proteins, mRNAs, miRNAs, lncRNAs, and DNA fragments. Their presence in biofluids makes them attractive candidates as innovative clinical diagnostic tools. However, the conventional isolation and analysis of high-purity exosomes in clinical application is challenging, with traditional methods facing a number of shortcomings, including low yield or purity, long periods of processing, high cost, and difficulties in standardization. In this study, we provide an overview of commonly used exosome isolation approaches with a focus on magnetic bead-based capture, an ideal methodology with high purity and integrality of exosomes. The current challenges on exosome isolation methods are also described to highlight areas for future research and development.
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