4.7 Review

Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks

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Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics

Stanislav Obruca et al.

Summary: This review summarizes the current knowledge on PHA accumulation in halophiles and thermophiles, highlighting the advantages of utilizing extremophiles for PHA production. It also discusses recent advances and future perspectives in metabolic engineering and synthetic biology for improving PHA production. The findings and ideas presented in this review suggest that biotechnological production of PHA by extremophiles can be sustainable and economically feasible, enabling PHA to compete with non-biodegradable petrochemical polymers in the market.

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Production of polyhydroxyalkanoates by three novel species of Marinobacterium

Meng-Ru Wang et al.

Summary: Several marine bacteria from the genus Marinobacterium were found to produce polyhydroxyalkanoates (PHA) using sugars and volatile fatty acids (VFAs) as carbon sources. Different species showed varying abilities in producing PHAs, with M. zhoushanense producing poly-3-hydroxybutyrate (PHB) from sucrose, M. nitratireducens from butyrate, and M. sediminicola showing tolerance to different VFAs and ability to produce PHBV with changeable compositions.

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Biosynthesis of diverse α,ω-diol-derived polyhydroxyalkanoates by engineered Halomonas bluephagenesis

Xu Yan et al.

Summary: Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible plastics potentially replacing petroleum-based plastics. This study established a synthesis pathway for 5-hydroxyvalerate (5HV) and produced various PHA polymers with different properties. Engineering H. bluephagenesis successfully scaled up the production of highly elastic and sticky PHA polymers for industrial applications.

METABOLIC ENGINEERING (2022)

Review Agricultural Engineering

Recent progress and challenges in microbial polyhydroxybutyrate (PHB) production from CO2 as a sustainable feedstock: A state-of-the-art review

Jiye Lee et al.

Summary: Recent progress in bacterial strains for PHB production from CO2, along with technical approaches like two-stage bioprocesses and bioelectrochemical systems, are discussed. Challenges and future perspectives for economically feasible PHB production are also addressed.

BIORESOURCE TECHNOLOGY (2021)

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Production of Polyhydroxyalkanoates (PHAs) by Vibrio alginolyticus Strains Isolated from Salt Fields

Hong-Fei Li et al.

Summary: Vibrio alginolyticus strains LHF01 and LHF02 isolated from salt fields can produce PHB from various sugars and organic acids. Optimization of NaCl concentration and culture temperature can improve PHB titer, while the use of propionate can result in the production of PHBV.

MOLECULES (2021)

Article Biochemical Research Methods

The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity

Amanda Pacholak et al.

Summary: This study investigated the biological response of Haloferax mediterranei to high salinity by analyzing protein expression using iTRAQ-based proteomic analysis. The differential protein expression under high salinity conditions resulted in differential production of PHAs and EPS. PHAs, as natural polymers with potential applications, are affected by the simultaneous synthesis of EPS.

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Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis

Xiao-Ran Jiang et al.

Summary: The study describes the engineering of Halomonas bluephagenesis to achieve high-level production of 3-hydroxypropionic acid (3HP) and its copolymer under open and unsterile conditions by deleting a newly identified degradation pathway and balancing redox state.

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Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli

Xuan Wang et al.

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NATURE COMMUNICATIONS (2021)

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Optimizing a Fed-Batch High-Density Fermentation Process for Medium Chain-Length Poly(3-Hydroxyalkanoates) in Escherichia coli

Ryan A. Scheel et al.

Summary: This study focused on the development and optimization of a fed-batch fermentation process in a stirred tank reactor for the biosynthesis of medium chain-length poly(3-hydroxyalkanoates) [PHA] polymers. By iteratively optimizing the semi-defined media composition and glucose feed rate, decanoic acid was successfully converted to poly(3-hydroxydecanoate) [PHD] at high yield, showcasing the potential of this method for controlling the production of PHA copolymers with specific compositions.

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Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2

Justin Panich et al.

Summary: The review discusses recent technological advances in utilizing metabolic engineering of the hydrogen-oxidizing bacterium Cupriavidus necator H16 to convert CO2 into biomass, particularly for biofuel production. Successes have been seen in biofuel production using this organism and through the implementation of electrolysis/artificial photosynthesis approaches for growth using renewable electricity and CO2. Future prospects include improving the suboptimal growth of C. necator in ambient concentrations of CO2.

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Fed-Batch mcl- Polyhydroxyalkanoates Production in Pseudomonas putida KT2440 and ΔphaZ Mutant on Biodiesel-Derived Crude Glycerol

Jose Manuel Borrero-de Acuna et al.

Summary: In this study, different fed-batch modes were utilized to synthesize mcl-PHAs from raw glycerol in P. putida strains, resulting in high production yields. The DO-stat approach was used to evaluate the PHA production ability of the strains, with the mutant Delta phaZ showing higher specific PHA volumetric productivity compared to the parental strain KT2440.

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Tailor-Made Polyhydroxyalkanoates by Reconstructing Pseudomonas Entomophila

Mengyi Li et al.

Summary: This study successfully reprogrammed Pseudomonas entomophila to synthesize PHA copolymers with both short- and medium/long-chain-length monomers. By optimizing the synthesis pathways, novel copolymers with high PHB content were produced, providing a new approach for the synthesis of tailor-made PHA polymers.

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Identification of regions affecting enzyme activity, substrate binding, dimer stabilization and polyhydroxyalkanoate (PHA) granule morphology in the PHA synthase of Aquitalea sp. USM4

Hui Lim et al.

Summary: The study demonstrated the importance of both the N- and C-termini of PhaC1(As) in regulating intracellular granule morphology, activity, substrate specificity, dimerization, and stability of the synthase.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Review Materials Science, Biomaterials

Additive manufacturing of polyhydroxyalkanoates (PHAs) biopolymers: Materials, printing techniques, and applications

Mehrshad Mehrpouya et al.

Summary: Additive manufacturing (AM) is a fast, reliable, and highly flexible solution for processing various materials and creating customized end-goods, with particular relevance placed on the use of biomaterials. Polyhydroxyalkanoates (PHAs) are emerging as a biobased and biodegradable material achievable through bacterial fermentation, with promising reports on manufacturing commodities in PHAs by additive manufacturing, although challenges remain for further expansion of their use.

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Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties

Ziyu Wang et al.

Summary: The bacterial outer membrane (OM) plays a crucial role in bioproduction, with OM-defected Halomonas bluephagenesis strains showing improved adaptation to lower salinity and increased production of bioproducts. The study highlights the significant impact of OM defection on metabolic engineering, synthetic biology studies, and industrial applications.

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Biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 by a Recombinant Cupriavidus necator

Kenji Tanaka et al.

Summary: PHBHHx is a practical bacterial polyhydroxyalkanoate produced by recombinant strains of Cupriavidus necator from CO2. The composition of PHBHHx can be controlled by using different enzymes, and its synthesis can be achieved using a mixture of H-2/O-2/CO2 and NH4)2SO4 as a nitrogen source.

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Synthetic Biology and Genome-Editing Tools for Improving PHA Metabolic Engineering

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Stimulus response-based fine-tuning of polyhydroxyalkanoate pathway in Halomonas

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Biosynthesis of functional polyhydroxyalkanoates by engineered Halomonas bluephagenesis

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A Nitrate-Blind P. putida Strain Boosts PHA Production in a Synthetic Mixed Culture

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Bacterial Polyesters: Microbial Polyhydroxyalkanoates and Nonnatural Polyesters (Adv. Mater. 35/2020)

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Rational flux-tuning of Halomonas bluephagenesis for co-production of bioplastic PHB and ectoine

Hong Ma et al.

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Polyhydroxyalkanoates (PHA) from Halomonas pacifica ASL10 and Halomonas salifodiane ASL11 isolated from Mariout salt lakes

Fady Abd El-malek et al.

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High PHA density fed-batch cultivation strategies for 4HB-rich P(3HB-co-4HB) copolymer production by transformant Cupriavidus malaysiensis USMAA1020

Hambali Norhafini et al.

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Production of medium chain length polyhydroxyalkanoate from acetate by engineered Pseudomonas putida KT2440

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Role of β-oxidation and de novo fatty acid synthesis in the production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa

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Utilization of waste fish oil and glycerol as carbon sources for polyhydroxyalkanoate production by Salinivibrio sp. M318

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Efficient production of polyhydroxyalkanoates (PHAs) from Pseudomonas mendocina PSU using a biodiesel liquid waste (BLW) as the sole carbon source

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Evaluation of medium-chain-length polyhydroxyalkanoate production by Pseudomonas putida LS46 using biodiesel by-product streams

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Microbial production of poly(hydroxybutyrate) from C1 carbon sources

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Optimization of polyhydroxybutyrate production by marine Bacillus megaterium MSBNO4 under solid state culture

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Adaptation of Cupriavidus necator to conditions favoring polyhydroxyalkanoate production

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