Elicitor Induced JA-Signaling Genes Are Associated with Partial Tolerance to Hemibiotrophic Pathogen Phytophthora capsici in Capsicum chinense

Phytophthora capsici causes root and stem rot disease in Capsicum. However, molecular mechanisms underlying this pathosystem are little known. The use of elicitors as tools that trigger defense responses to biotic stresses to study molecular plant defense has increased. In this study, early defense induced in the susceptible cultivar C. chinense using three elicitors to assess its role during interaction with hemibiotrophic P. capsici. The response to infection by phenotypic analyses across the time during disease development in seedlings treated with elicitors was compared. Likewise; defense-gene expression were investigated by qRT-PCR. A total of five resistance genes were used as markers of signaling pathways mediated by jasmonate/ethylene (JA/ET) and salicylic acid (SA). Further, six R genes analogs (CcRGAS) related to oomycete-defense were employed. The results showed that elicitors MeJA and b-aminobutyric acid (BABA) slightly reduced disease symptoms. Moreover, MeJA or BABA treatments followed by challenge with P. capsici up-regulated the expression level of genes related to the JA/ET signaling pathway (CcLOX2, CcPDF1 and CcETR1). Furthermore, MeJA treatment followed by challenge triggered a significant induction of de CcRGAS and CcRPP13 expression within 24 h of inoculation. This suggests that in the early defense mechanisms against P. capsici JA signaling plays an important role.

Automated summary

This study investigated the molecular mechanisms underlying the interaction between Phytophthora capsici and Capsicum chinense, a susceptible cultivar. Three elicitors were used to induce early defense in the plants, and the expression of defense-related genes was analyzed. The results showed that MeJA and BABA elicitors reduced disease symptoms and up-regulated genes related to the JA/ET signaling pathway. This suggests that JA signaling plays an important role in the early defense against P. capsici.