Impact of Azospirillum sp. B510 on the Rhizosphere Microbiome of Rice under Field Conditions

There has been increasing attention toward the influence of biofertilizers on the composition of microbial communities associated with crop plants. We investigated the impact of Azospirillum sp. B510, a bacterial strain with nitrogen-fixing ability, on the structure of bacterial and fungal communities within rice plant rhizospheres by amplicon sequencing at two sampling stages (the vegetative and harvest stages of rice). Principal coordinate analysis (PCoA) demonstrated a significant community shift in the bacterial microbiome when the plants were inoculated with B510 at the vegetative stage, which was very similar to the effect of chemical N-fertilizer application. This result suggested that the inoculation with B510 strongly influenced nitrogen uptake by the host plants under low nitrogen conditions. Least discriminant analysis (LDA) showed that the B510 inoculation significantly increased the N-2-fixing Clostridium, Aeromonas and Bacillus populations. In contrast, there was no apparent influence of B510 on the fungal community structure. The putative functional properties of bacteria were identified through PICRUSt2, and this hinted that amino acid, sugar and vitamin production might be related to B510 inoculation. Our results indicate that B510 inoculation influenced the bacterial community structure by recruiting other N-2-fixing bacteria in the absence of nitrogen fertilizer.

Automated summary

This study investigated the influence of Azospirillum sp. B510 on the structure of bacterial and fungal communities within rice plant rhizospheres. The results showed that the bacterial community experienced significant changes after inoculation with B510 at the vegetative stage, while the fungal community structure remained unaffected. Inoculation with B510 increased the populations of nitrogen-fixing bacteria and promoted nitrogen uptake in rice plants.