期刊
CELLS
卷 11, 期 16, 页码 -出版社
MDPI
DOI: 10.3390/cells11162502
关键词
rose bengal; glioblastoma; photosensitizers; nanomedicine; drug delivery
类别
资金
- Italian Ministry of Education, University and Research (MIUR): Dipartimenti di Eccellenza Program (2018-2022), Dept. of Biology and Biotechnology L. Spallanzani, University of Pavia
The study developed a combined scheme to enhance the uptake of Rose bengal (RB) in astrocytoma cells and reduce its extracellular release, thereby improving the effectiveness of photodynamic therapy (PDT).
Photodynamic therapy (PDT) is a promising anticancer strategy based on the light energy stimulation of photosensitizers (PS) molecules within a malignant cell. Among a multitude of recently challenged PS, Rose bengal (RB) has been already reported as an inducer of cytotoxicity in different tumor cells. However, RB displays a low penetration capability across cell membranes. We have therefore developed a short-term amino acids starvation protocol that significantly increases RB uptake in human astrocytoma cells compared to normal rat astrocytes. Following induced starvation uptake, RB is released outside cells by the exocytosis of extracellular vesicles (EVs). Thus, we have introduced a specific pharmacological treatment, based on the GW4869 exosomes inhibitor, to interfere with RB extracellular release. These combined treatments allow significantly reduced nanomolar amounts of administered RB and a decrease in the time interval required for PDT stimulation. The overall conditions affected astrocytoma viability through the activation of apoptotic pathways. In conclusion, we have developed for the first time a combined scheme to simultaneously increase the RB uptake in human astrocytoma cells, reduce the extracellular release of the drug by EVs, and improve the effectiveness of PDT-based treatments. Importantly, this strategy might be a valuable approach to efficiently deliver other PS or chemotherapeutic drugs in tumor cells.
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