期刊
CELLS
卷 11, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/cells11121965
关键词
CD3 antigen; exosomes; immune capture; T lymphocytes; metabolomics; proteomics; small extracellular vesicles
类别
资金
- National Science Centre, Poland [2016/22/M/NZ5/00667]
- NIH [R01-CA168628, U01-DE029759]
This study demonstrates the isolation of CD3(+) exosomes from human plasma using immune capture and their subsequent molecular profiling using proteomics and metabolomics. The CD3(+) exosomes were enriched with proteins and metabolites that reflect the molecular features of T lymphocytes.
Exosomes that are released by T cells are key messengers involved in immune regulation. However, the molecular profiling of these vesicles, which is necessary for understanding their functions, requires their isolation from a very heterogeneous mixture of extracellular vesicles that are present in the human plasma. It has been shown that exosomes that are produced by T cells could be isolated from plasma by immune capture using antibodies that target the CD3 antigen, which is a key component of the TCR complex that is present in all T lymphocytes. Here, we demonstrate that CD3(+) exosomes that are isolated from plasma can be used for high-throughput molecular profiling using proteomics and metabolomics tools. This profiling allowed for the identification of proteins and metabolites that differentiated the CD3(+) from the CD3(-) exosome fractions that were present in the plasma of healthy donors. Importantly, the proteins and metabolites that accumulated in the CD3(+) vesicles reflected the known molecular features of T lymphocytes. Hence, CD3(+) exosomes that are isolated from human plasma by immune capture could serve as a T cell biopsy.
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