期刊
CELLS
卷 11, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/cells11142156
关键词
keratinocyte; stem cells; regeneration; homeostasis; interfollicular epidermis; integrin
类别
资金
- Fondation Gaydoul
- Swiss National Science Foundation (SNSF) [205321_179012, 33IC30_180418, 316030_205706]
- Olga Mayenfisch Stiftung
- University Medicine Zurich
- Swiss National Science Foundation (SNF) [316030_205706, 205321_179012, 33IC30_180418] Funding Source: Swiss National Science Foundation (SNF)
This study found that human suprabasal keratinocytes have proliferative capacity and can retro-differentiate into basal stem cells in a wound situation or when in contact with the basement membrane.
The basal layer of human interfollicular epidermis has been described to harbour both quiescent keratinocyte stem cells and a transit amplifying cell population that maintains the suprabasal epidermal layers. We performed immunofluorescence analyses and revealed that the main proliferative keratinocyte pool in vivo resides suprabasally. We isolated from the human epidermis two distinct cell populations, the basal and the suprabasal keratinocytes, according to the expression of integrin beta 4 (i beta 4). We compared basal i beta 4(+) or suprabasal i beta 4(-) keratinocytes with respect to their proliferation and colony-forming ability and their Raman spectral properties. In addition, we generated dermo-epidermal substitutes using freshly isolated and sorted basal i beta 4(+) or suprabasal i beta 4(-) keratinocytes and transplanted them on immuno-compromised rats. We show that suprabasal i beta 4(-) keratinocytes acquire a similar proliferative capacity as basal i beta 4(+) keratinocytes after two weeks of culture in vitro, with expression of high levels of i beta 4 and downregulation of K10 expression. In addition, both basal i beta 4(+) and suprabasal i beta 4(-) keratinocytes acquire authentic self-renewing properties during the in vitro 3D-culture phase and are able to generate and maintain a fully stratified epidermis for 16 weeks in vivo. Therefore, against the leading dogma, we propose that human suprabasal keratinocytes can retro-differentiate into true basal stem cells in a wound situation and/or when in contact with the basement membrane.
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