期刊
SCIENCE ADVANCES
卷 8, 期 28, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abo5738
关键词
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资金
- IWT-FISCH-SBO project ARBOREF [140894]
- iBOF project NextBioRef
- Research Foundation Flanders (FWO)
- FWO [G011620N, G020618N]
- Institute for the promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
- DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) [DE-SC0018409]
To improve the conversion efficiency of lignocellulosic biomass, researchers incorporated scopoletin, a compound, into the lignin polymer of Arabidopsis through genetic modification. The transgenic plants showed higher saccharification efficiency without affecting plant growth.
Lignin is the main factor limiting the enzymatic conversion of lignocellulosic biomass into fermentable sugars. To reduce the recalcitrance engendered by the lignin polymer, the coumarin scopoletin was incorporated into the lignin polymer through the simultaneous expression of FERULOYL-CoA 6'-HYDROXYLASE 1 (F6'H1) and COUMARIN SYNTHASE (COSY) in lignifying cells in Arabidopsis. The transgenic lines overproduced scopoletin and incorporated it into the lignin polymer, without adversely affecting plant growth. About 3.3% of the lignin units in the transgenic lines were derived from scopoletin, thereby exceeding the levels of the traditional p-hydroxyphenyl units. Saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was 40% higher than for wild type.
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