期刊
ACS SENSORS
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.2c00474
关键词
Single cell; secretion; bioanalysis; optical sensing; fluorescence; surface plasmon resonance; surface-enhanced Raman scattering
资金
- National Natural Science Foundation of China [21934001, 21974088]
- Science and Technology Commission of Shanghai Municipality [21ZR1481300]
Innovation in single-cell analysis has enabled accurate sensing of cell secretion events with high spatiotemporal resolution, revolutionizing the analysis of cell secretion. Optical sensing strategies allow for real-time monitoring of various substances released by single live cells, providing high-resolution analysis capabilities with accuracy.
Measuring cell secretion events is crucial to understand the fundamental cell biology that underlies cell-cell communication, migration, proliferation, and differentiation. Although strategies targeting cell populations have provided significant information about live cell secretion, they yield ensemble profiles that obscure intrinsic cell-to-cell variations. Innovation in single-cell analysis has made breakthroughs allowing accurate sensing of a wide variety of secretions and their release dynamics with high spatiotemporal resolution. This perspective focuses on the power of single-cell protocols to revolutionize cell-secretion analysis by allowing real-time and real-space measurements on single live cell resolution. We begin by discussing recent progress on single-cell bioanalytical techniques, specifically optical sensing strategies such as fluorescence-, surface plasmon resonance-, and surface-enhanced Raman scattering-based strategies, capable of in situ real-time monitoring of single-cell released ions, metabolites, proteins, and vesicles. Single-cell sensing platforms which allow for high-throughput high-resolution analysis with enough accuracy are highlighted. Furthermore, we discuss remaining challenges that should be addressed to get a more comprehensive understanding of secretion biology. Finally, future opportunities and potential breakthroughs in secretome analysis that will arise as a result of further development of single-cell sensing approaches are discussed.
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