期刊
MOLECULAR IMAGING
卷 2022, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2022/7456365
关键词
-
资金
- National Natural Science Foundation of China [81501499]
- Shanghai Municipal Key Clinical Specialty [shslczdzk03403]
This study demonstrates that radioiodine-labeled Var3-pHLIP effectively targets BC cells in an acidic tumor microenvironment and inhibits the growth of breast cancer xenografts through ionizing radiation.
Purpose. Extracellular acidity is a marker of highly aggressive breast cancer (BC). pH-low insertion peptides (pHLIPs) target the acidic tumor microenvironment. This study evaluates the distribution and therapeutic efficacy of radioiodine-labeled pHLIP variant 3 (Var3) in a mouse model of BC. Methods. The binding of fluorescein isothiocyanate (FITC)- or radioiodine-125 (I-125) labeled Var3-pHLIP to MDA-MB-231, 4T1, and SK-BR-3 BC cell lines under different pH values was evaluated in vitro. The distribution of I-125-labeled Var3-pHLIP and wild-type- (WT-) pHLIP in tumor-bearing mice was analyzed in vivo using micro-SPECT/CT imaging. The therapeutic efficacy of radioiodine-131 (I-131)-labeled Var3-pHLIP in MDA-MB-231 xenografts was evaluated by relative tumor volume measurement and immunohistochemical analysis. Results. The binding ability of FITC- or I-125-labeled Var3-pHLIP to tumor cells increased with the decrease in pH. The tumor-to-background ratio of I-125-Var3-pHLIP in BC xenografts showed the best imaging contrast at 24 h or 48 h postinjection. The uptake of I-125-Var3-pHLIP in MDA-MB-231 xenografts at 2 h postinjection was significantly higher than that of I-125-WT-pHLIP (3.76 & PLUSMN;0.37 vs. 2.87 & PLUSMN;0.60%ID/g, p=0.046). The relative tumor volume in MDA-MB-231 xenografts was significantly lower in the I-131-Var3-pHLIP-treated group than in the groups treated with Var3-pHLIP (p=0.027), I-131 (p=0.001), and saline (p < 0.001). The I-131-Var 3-pHLIP group presented a lower expression of Ki67 and a higher expression of caspase 3. Conclusion. Radioiodine-labeled Var3-pHLIP effectively targeted BC cells in an acidic environment and inhibited the growth of MDA-MB-231 xenografts by ionizing radiation.
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