期刊
FOOD BIOSCIENCE
卷 48, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fbio.2022.101737
关键词
Whey protein hydrolysates; Purification; ACE inhibition; Molecular docking
资金
- Milkyway Import Export Corp.
ACE inhibitory peptides were detected in whey protein hydrolysate, which may provide novel ACE inhibitors for hypertension management.
Angiotensin I-converting enzyme (ACE) plays a significant role in the regulation of blood pressure via generating angiotensin II (Ang II). Using natural inhibitors to block the activity of ACE is an alternative method to minimize the side effects of commercial drugs, and is a major goal of hypertension management. This study detected the presence of ACE inhibitory peptides in whey protein hydrolysate. The whey protein hydrolysate was separated sequentially by ultrafiltration and RP-HPLC. LC-MS/MS revealed the amino acid sequences of the fractions and four potential ACE inhibitory peptides were selected (PQVSTPTL, MPGP, PMHIR, PPLT) for synthesis. IC50 values of these four peptides were 86 +/- 8, 179 +/- 4, 90 +/- 6, and 168 +/- 4 mu M, respectively. Then, molecular docking analysis was used to assess how these peptides interact with ACE. PQVSTPTL exhibited the lowest binding energy (similar to 6.64 kcal/mol) with the ACE molecule. These four reported peptides were with no allergenicity nor toxicity. These results indicated that whey protein hydrolysate could be a suitable resource for obtaining novel inhibitory peptides against ACE and provides information for obtaining novel ACE inhibitors for hypertension management.
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