4.5 Article

Stability of novel urinary biomarkers used for lupus nephritis

期刊

FRONTIERS IN PEDIATRICS
卷 10, 期 -, 页码 -

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FRONTIERS MEDIA SA
DOI: 10.3389/fped.2022.974049

关键词

lupus nephritis; urine; biomarker; stability; SLE

资金

  1. Lupus Foundation of America
  2. Falk Foundation
  3. Portico
  4. NIH [P30AR076316]
  5. [P50DK096418]

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In this study, the stability of RAIL biomarkers used to monitor lupus nephritis activity in children was tested. The results showed that these biomarkers can be stored for a short period of time under clinically relevant conditions.
BackgroundThe Renal Activity Index for Lupus (RAIL) is a composite score of six urinary biomarkers (neutrophil gelatinase-associated lipocalin (NGAL), monocyte chemoattractant protein-1 (MCP-1), kidney injury molecule-1 (KIM-1), ceruloplasmin, adiponectin, and hemopexin) used to monitor lupus nephritis activity in children. We tested stability of RAIL biomarkers prior to meaningful clinical use. MethodsUrine samples were tested by ELISA under shipping conditions, freeze/thaw, ambient and longer-term storage. Statistical analysis was performed via Deming Regression, Bland-Altman and Spearman Correlation Coefficient. ResultsBiomarker concentration were comparable to freshly collected urine following storage at -80 degrees C for up to 3 months, and at 4 or 25 degrees C up to 48 h followed by -80 degrees C. Neither shipping on dry or wet ice exposure nor addition of two freeze-thaw cycles led to loss of signal, with excellent Spearman Correlation coefficients under all conditions. ConclusionsRAIL biomarkers are stable following short-term storage at clinically relevant conditions.

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