A chromosome-level genome assembly of the jade perch (Scortum barcoo)

Endemic to Australia, jade perch (Scortum barcoo) is a highly profitable freshwater bass species. It has extraordinarily high levels of omega-3 polyunsaturated fatty acids (PUFAs), which detailed genes involved in are largely unclear. Meanwhile, there were four chromosome-level bass species have been previous sequenced, while the bass ancestor genome karyotypes have not been estimated. Therefore, we sequenced, assembled and annotated a genome of jade perch to characterize the detailed genes for biosynthesis of omega-3 PUFAs and to deduce the bass ancestor genome karyotypes. We constructed a chromosome-level genome assembly with 24 pairs of chromosomes, 657.7 Mb in total length, and the contig and the scaffold N50 of 4.8 Mb and 28.6 Mb respectively. We also identified repetitive elements (accounting for 19.7% of the genome assembly) and predicted 26,905 protein-coding genes. Meanwhile, we performed genome-wide localization and characterization of several important genes encoding some key enzymes in the biosynthesis pathway of PUFAs. These genes may contribute to the high concentration of omega-3 in jade perch. Moreover, we conducted a series of comparative genomic analyses among four representative bass species at a chromosome level, resulting in a series of sequences of a deductive bass ancestor genome.

Automated summary

In this study, we sequenced the genome of the jade perch, a highly profitable freshwater bass species endemic to Australia, to identify the genes involved in the biosynthesis of omega-3 polyunsaturated fatty acids (PUFAs) and to deduce the ancestor genome karyotypes of bass. We constructed a chromosome-level genome assembly and identified repetitive elements and protein-coding genes. We also characterized key genes in the PUFA biosynthesis pathway and performed comparative genomic analyses among different bass species, resulting in sequences of a deductive bass ancestor genome.