期刊
REDOX BIOLOGY
卷 53, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.redox.2022.102335
关键词
Aging; Mitochondria; Reactive oxygen species; C; elegans; Antioxidant; Thioredoxin
资金
- National Institute of General Medical Sciences (NIGMS) [R01 GM121756]
- Canadian Institutes of Health Research (CIHR)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Fonds de Recherche du Quebec Sante (FRQS)
- Parkinson Quebec
- NSERC
- FRQS
Mild impairment of mitochondrial function can increase lifespan in genetic model organisms. The upregulation of genes involved in the mitochondrial thioredoxin system, mediated by the mitochondrial unfolded protein response, is specifically required for the longevity of mitochondrial mutants. The mitochondrial thioredoxin system also plays a role in stress resistance.
Mild impairment of mitochondrial function has been shown to increase lifespan in genetic model organisms including worms, flies and mice. To better understand the mechanisms involved, we analyzed RNA sequencing data and found that genes involved in the mitochondrial thioredoxin system, trx-2 and trxr-2, are specifically upregulated in long-lived mitochondrial mutants but not other non-mitochondrial, long-lived mutants. Upregulation of trx-2 and trxr-2 is mediated by activation of the mitochondrial unfolded protein response (mitoUPR). While we decided to focus on the genes of the mitochondrial thioredoxin system for this paper, we identified multiple other antioxidant genes that are upregulated by the mitoUPR in the long-lived mitochondrial mutants including sod-3, prdx-3, gpx-6, gpx-7, gpx-8 and glrx-5. In exploring the role of the mitochondrial thioredoxin system in the long-lived mitochondrial mutants, nuo-6 and isp-1, we found that disruption of either trx-2 or trxr-2 significantly decreases their long lifespan, but has no effect on wild-type lifespan, indicating that the mitochondrial thioredoxin system is specifically required for their longevity. In contrast, disruption of the cytoplasmic thioredoxin gene trx-1 decreases lifespan in nuo-6, isp-1 and wild-type worms, indicating a non-specific detrimental effect on longevity. Disruption of trx-2 or trxr-2 also decreases the enhanced resistance to stress in nuo-6 and isp-1 worms, indicating a role for the mitochondrial thioredoxin system in protecting against exogenous stressors. Overall, this work demonstrates an important role for the mitochondrial thioredoxin system in both stress resistance and lifespan resulting from mild impairment of mitochondrial function.
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