Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation

Background: Intestinal inflammation is prevalent in chicken, which results in decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota and chicken growth performance. However, whether gut microbiota impacts chicken growth performance by lessening intestinal inflammation remains elusive. Results: Seven-weeks-old male and female chickens with the highest or lowest body weights were significantly different in breast and leg muscle indices and average cross-sectional area of muscle cells. 16S rRNA gene sequencing indicated Gram-positive bacteria, such as Lactobacilli, were the predominant species in high body weight chickens. Conversely, Gram-negative bacteria, such as Comamonas, Acinetobacter, Brucella, Escherichia-Shigella, Thermus, Undibacterium, and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium were significantly abundant in low body weight chickens. Serum lipopolysaccharide (LPS) level was significantly higher in low body weight chickens (101.58 +/- 5.78 ng/mL) compared with high body weight chickens (85.12 +/- 4.79 ng/mL). The expression of TLR4, NF-kappa B, MyD88, and related inflammatory cytokines in the jejunum was significantly upregulated in low body weight chickens, which led to the damage of gut barrier integrity. Furthermore, transferring fecal microbiota from adult chickens with high body weight into 1-day-old chicks reshaped the jejunal microbiota, mitigated inflammatory response, and improved chicken growth performance. Conclusions: Our findings suggested that jejunal microbiota could affect chicken growth performance by mitigating intestinal inflammation.

Automated summary

This study found that jejunal microbiota can affect chicken growth performance by alleviating intestinal inflammation. Gram-positive bacteria were predominant in high body weight chickens, while Gram-negative bacteria were abundant in low body weight chickens. Low body weight chickens had higher levels of serum lipopolysaccharide and upregulated expression of inflammatory cytokines in the jejunum.