期刊
METHODS AND APPLICATIONS IN FLUORESCENCE
卷 10, 期 4, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/2050-6120/ac7bd8
关键词
review; nucleosides; DNA; fluorescent probes; nucleoside analogues; DNA-binding proteins
资金
- French National Centre for Scientific Research (CNRS)
- University of Strasbourg
Fluorescent nucleoside analogues (FNAs) are important tools in studying the interactions of nucleic acids with nucleic acid-binding proteins. They enable real-time monitoring of binding interactions in solutions, with high sensitivity. FNAs are also used to study the conformational dynamics of biomolecular complexes. Therefore, FNAs have potential applications in fundamental biological research and molecular diagnostics drug discovery.
Fluorescent nucleoside analogues (FNAs) are indispensable in studying the interactions of nucleic acids with nucleic acid-binding proteins. By replacing one of the poorly emissive natural nucleosides, FNAs enable real-time optical monitoring of the binding interactions in solutions, under physiologically relevant conditions, with high sensitivity. Besides that, FNAs are widely used to probe conformational dynamics of biomolecular complexes using time-resolved fluorescence methods. Because of that, FNAs are tools of high utility for fundamental biological research, with potential applications in molecular diagnostics and drug discovery. Here I review the structural and physical factors that can be used for the conversion of the molecular binding events into a detectable fluorescence output. Typical environmentally sensitive FNAs, their properties and applications, and future challenges in the field are discussed.
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