期刊
METHODS AND APPLICATIONS IN FLUORESCENCE
卷 10, 期 3, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/2050-6120/ac7944
关键词
fluorescent probe; S; O-CNQDs; Cu2+; quenching mechanism; cell imaging
资金
- National Natural Science Foundation of China [21874087]
- Natural Science Foundation of Shanxi Province of China [201901D111210, 201901D111459]
- Key Research Project of Science and Technology in JinZhong-Social Development Projects [Y213003]
- Special Project of Lvliang for Introduced High-level Science and Technology Talents [2021RC-2-1]
In this study, fluorescent sulfur and oxygen co-doped graphitic carbon nitride quantum dots (S,O-CNQDs) were prepared and utilized as a probe for the detection of Cu2+. The S,O-CNQDs exhibited efficient fluorescence quenching by Cu2+ under optimum conditions, enabling their application in various samples such as tap water, lake water, human serum, urine, and living cells. The as-prepared S,O-CNQDs showed excellent properties and have potential for biological applications.
A fluorescent sulfur and oxygen co-doped graphitic carbon nitride quantum dots (S,O-CNQDs) were prepared from ethylenediaminetetraacetic acid disodium salt dihydrate and thiourea as the carbon and sulfur sources. The morphology and surface functional groups of S,O-CNQDs were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The fluorescence of S,O-CNQDs could be quenched efficiently by Cu2+ under the optimum conditions. The S,O-CNQDs could function as an excellent fluorescent probe for Cu2+ detection with a wide linear range of 0.50-15 mu M and a low detection limit of 0.58 nM. In addition, this fluorescent probe was employed for monitoring Cu2+ in samples of tap water, lake water, human serum and urine with good recoveries from 99.0% to 110.0%. Moreover, the S,O-CNQDs with high cell penetration and low cytotoxicity were utilized for Cu2+ detection in living cells. Owing to the excellent properties of S,O-CNQDs, the as-prepared S,O-CNQDs can be a potential candidate for biological applications.
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