期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.911024
关键词
rotavirus; in vivo imaging system; transmission; Nano-luciferase; tissue tropism
类别
资金
- National Institutes of Health (NIH) [R01 AI150796, R56 AI167285, R01 AI125249, U19 AI116484]
- VA Merit Grant [GRH0022]
In this study, a recombinant murine-like rotavirus (RV) encoding a Nano-Luciferase reporter was generated and used to track the replication and transmission dynamics of the virus in vivo. The NLuc-expressing RV showed replication competence in cell culture and virulence in neonatal mice. Noninvasive imaging revealed that RV replication peaked at days 2 to 5 post infection, and transmission to uninoculated littermates occurred as early as 3 days post infection. The study provides a valuable tool for studying tissue tropism and factors regulating RV replication and spread.
Rotaviruses (RVs) are one of the main causes of severe gastroenteritis, diarrhea, and death in children and young animals. While suckling mice prove to be highly useful small animal models of RV infection and pathogenesis, direct visualization tools are lacking to track the temporal dynamics of RV replication and transmissibility in vivo. Here, we report the generation of the first recombinant murine-like RV that encodes a Nano-Luciferase reporter (NLuc) using a newly optimized RV reverse genetics system. The NLuc-expressing RV was replication-competent in cell culture and both infectious and virulent in neonatal mice in vivo. Strong luciferase signals were detected in the proximal and distal small intestines, colon, and mesenteric lymph nodes. We showed, via a noninvasive in vivo imaging system, that RV intestinal replication peaked at days 2 to 5 post infection. Moreover, we successfully tracked RV transmission to uninoculated littermates as early as 3 days post infection, 1 day prior to clinically apparent diarrhea and 3 days prior to detectable fecal RV shedding in the uninoculated littermates. We also observed significantly increased viral replication in Stat1 knockout mice that lack the host interferon signaling. Our results suggest that the NLuc murine-like RV represents a non-lethal powerful tool for the studies of tissue tropism and host and viral factors that regulate RV replication and spread, as well as provides a new tool to facilitate the testing of prophylactic and therapeutic interventions in the future.
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