期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.860327
关键词
endothelial (dys)function; inflammation; immune mediated disorders; CD4+memory T cells; NF-kB signaling; cellular interaction; transendothelial migration
类别
资金
- Dutch Arthritis Society [RF16-1-302]
- LSBR [1649]
- ZonMW NWO Vici [91819632]
The study shows that CD4(+) memory T cells can induce inflammatory responses in endothelial cells, and inhibition of NF-kappa B signaling can modulate these responses. Moreover, pharmacological inhibitors of NF-kappa B signaling can potentiate the effects of current therapies for IMIDs on endothelial cell inflammation.
Endothelial cells (ECs) are important contributors to inflammation in immune-mediated inflammatory diseases (IMIDs). In this study, we examined whether CD4(+) memory T (T-m) cells can drive EC inflammatory responses. Human T-m cells produced ligands that induced inflammatory responses in human umbilical vein EC as exemplified by increased expression of inflammatory mediators including chemokines and adhesion molecules. NF-kappa B, a key regulator of EC activation, was induced by T-m cell ligands. We dissected the relative contribution of canonical and non-canonical NF-kappa B signaling to T-m induced EC responses using pharmacological small molecule inhibitors of IKK beta (iIKK beta) or NF-kappa B inducing kinase (iNIK). RNA sequencing revealed substantial overlap in IKK beta and NIK regulated genes (n=549) that were involved in inflammatory and immune responses, including cytokines (IL-1 beta, IL-6, GM-CSF) and chemokines (CXCL5, CXCL1). NIK regulated genes were more restricted, as 332 genes were uniquely affected by iNIK versus 749 genes by iIKK beta, the latter including genes involved in metabolism, proliferation and leukocyte adhesion (VCAM-1, ICAM-1). The functional importance of NIK and IKK beta in EC activation was confirmed by transendothelial migration assays with neutrophils, demonstrating stronger inhibitory effects of iIKK beta compared to iNIK. Importantly, iIKK beta - and to some extent iNIK - potentiated the effects of currently employed therapies for IMIDs, like JAK inhibitors and anti-IL-17 antibodies, on EC inflammatory responses. These data demonstrate that inhibition of NF-kappa B signaling results in modulation of T-m cell-induced EC responses and highlight the potential of small molecule NF-kappa B inhibitors as a novel treatment strategy to target EC inflammatory responses in IMIDs.
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