期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.935692
关键词
sulforaphane; macrophages; M1 polarization; glycolysis; PKM2; interleukin 1 beta
类别
资金
- FWF (Austrian Science Fund) [P32600]
- University of Vienna
- CSC fellowship
- Austrian Science Fund (FWF) [P32600] Funding Source: Austrian Science Fund (FWF)
This study reveals that sulforaphane (Sfn) inhibits the expression of proinflammatory markers in M1 macrophages and enhances cellular energy levels. By impeding the function of pyruvate kinase M2 (PKM2), Sfn alters the glycolytic activity and IL-1β expression in macrophages, thus influencing the inflammatory response.
Murine macrophages activated by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) polarize to the M1 type by inducing proinflammatory marker proteins and changing their energy metabolism to increased aerobic glycolysis and reduced respiration. We here show that the aliphatic isothiocyanate sulforaphane (Sfn) diminishes M1 marker expression (IL-1 beta, IL-6, TNF-alpha, iNOS, NO, and ROS) and leads to highly energetic cells characterized by both high glycolytic and high respiratory activity as assessed by extracellular flux analysis. Focusing on a potential connection between high glycolytic activity and low IL-1 beta expression in M1 (LPS/Sfn) macrophages, we reveal that Sfn impedes the moonlighting function of pyruvate kinase M2 (PKM2) in M1 macrophages. Sfn limits mono/dimerization and nuclear residence of PKM2 accompanied by reduced HIF-1 alpha levels, Stat3 phosphorylation at tyrosine 705, and IL-1 beta expression while preserving high levels of cytosolic PKM2 tetramer with high glycolytic enzyme activity. Sfn prevents glutathionylation of PKM2 in LPS-stimulated macrophages which may account for the reduced loss of PKM2 tetramer. Overall, we uncover PKM2 as a novel affected hub within the anti-inflammatory activity profile of Sfn.
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