4.6 Article

Constitutive High Expression Level of a Synthetic Deleted Encoding Gene of Talaromyces minioluteus Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in Komagataella phaffii (Pichia pastoris)

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Summary: The gene construct encoding Talaromyces minioluteus dextranase (r-TmDEX) was expressed in Pichia pastoris with an increase in transgene dosage resulting in higher extracellular yield of the enzyme. The optimal activity of the N-glycosylated r-TmDEX was observed at pH 4.5-5.5 and temperature 50-60 degrees C, while the addition of sucrose as a stabilizer maintained enzyme activity under harsh conditions. The use of the P. pastoris pGAP-based expression system proved to be suitable for large-scale production of dextranase and its application in dextran removal.

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