Antioxidant and Enzyme Inhibitory Properties, and HPLC-MS/MS Profiles of Different Extracts of Arabis carduchorum Boiss.: An Endemic Plant to Turkey

In this study, six extracts of Arabis carduchorum prepared from solvents of varying polarity (water, methanol, ethyl acetate, dichloromethane, and n-hexane) were investigated for their chemical profiles and total phenolic and flavonoid contents (TPC/TFC) using HPLC-MS/MS and spectrophotometric/colorimetric assays, respectively, along with the assessment of their antioxidant and enzyme inhibitory effects. In general, the polar extracts (methanol, water, and infusion) showed higher TPC/TFC than the other extracts (range: 13.73-26.62 mg GAE/g; 2.66-52.95 mg RE/g, respectively). The total antioxidant capacity of the polar extracts ranged from 0.90-1.85 mmol TE/g in phosphomolybdenum assay, while the free radical scavenging potential ranged from 4.36-32.13 mg TE/g and 12.58-67.73 mg TE/g in DPPH and ABTS assays, respectively. While the water and infuse extract inhibited only butyrylcholinesterase (2.68 and 2.39 mg GALAE/g, respectively), the other extracts were found to inhibit both acetyl- and butyryl-cholinesterases (0.96-2.73 mg GALAE/g and 1.86-5.11 mg GALAE/g, respectively). On the contrary, all extracts exhibited anti-tyrosinase (40.24-59.22 mg KAE/g) and anti-amylase (0.09-0.63 mmol ACAE/g) activities. Overall, this study highlighted the effect of solvent polarity on the extraction of bioactive compounds and the biological properties of the extracts of A. carduchorum; hence, the results obtained could be used to guide optimum extraction in future studies.

Automated summary

This study compared the chemical profiles, total phenolic and flavonoid contents, as well as antioxidant and enzyme inhibitory effects of extracts of Arabis carduchorum prepared from solvents of varying polarity. The polar extracts showed higher antioxidant capacity, while all extracts exhibited enzyme inhibitory activities.