期刊
PEERJ
卷 10, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.13745
关键词
Cancer stem cells; Cervical squamous cell carcinoma; Epithelial-mesenchymal transition; Tumor budding
资金
- Medical Scientific Research Foundation of Guangdong Province, China [A2019318]
- Scientific Research and Cultivation Project of Meizhou People's Hospital, China [PY-C2021008]
- Social Development Science and Technology project of Meizhou, China [2021B83]
Recent evidence suggests that cancer stem cells (CSCs) are the origin of cancers. This study investigated the relationship between tumor buds, which are hypothesized to represent epithelial-mesenchymal transition (EMT)-like CSCs, and EMT and CSC markers in cervical squamous cell carcinoma (CxSCC). The findings indicate that tumor buds in CxSCC may represent EMT-like CSCs in vivo.
Recent evidence indicates that cancer stem cells (CSCs) are the origin of cancers. Scientists have identified CSCs in various tumors and have suggested the existence of a variety of states of CSCs. The existence of epithelial-mesenchymal transition (EMT)-like CSCs has been confirmed in vitro, but they have not been identified in vivo. Tumor budding was defined as single cell or clusters of <= 5 cells at the invasive front of cancers. Such tumor budding is hypothesized to be closely related to EMT and linked to CSCs, especially to those migrating at the invasive front. Therefore, tumor budding has been proposed to represent EMT-like stem cells. However, this hypothesis has not yet been proven. Thus, we studied the expression of EMT markers, certain CSC markers of tumor budding, and the tumor center of cervical squamous cell carcinoma (CxSCC). We performed tissue chip analyses of 95 primary CxSCCs from patients. Expression of EMT and CSC markers (E-cadherin, beta-catenin, vimentin, Ki67, CD44, SOX2 , and ALDH1A1) in a set of tumor samples on tissue chips (87 cases of tumor budding/the main tumor body) were evaluated by immunohistochemistry. We found that the cell-membranous expression of beta -catenin was stronger in the main tumor body than in tumor buds. Compared with the main tumor body, tumor buds had reduced proliferative activity as measured by Ki67. Moreover, vimentin expression was high and E-cadherin expression was low in tumor buds. Expression of EMT-related markers suggested that tumor buds were correlated with EMT. We noted that CxSCC tumor buds had a CD44(negative/low)/SOX2(high)/ALDH1A1(high) staining pattern, indicating that tumor buds of CxSCC present CSC-like immunophenotypic features. Taken together, our data indicate that tumor buds in CxSCC may represent EMT-like CSCs in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据