期刊
PEERJ
卷 10, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.13778
关键词
Fasciola hepatica; LAMP; Environment; Fluke; Parasite; Field; Detection
资金
- Cooperative Research Centres Project (CRC-P)
- Australian Research Training Program scholarship
- Tim Healey Memorial Scholarship - Primary Industries South Australia (PIRSA)
Fasciola hepatica, commonly known as liver flukes, is a significant parasitic disease affecting humans and livestock worldwide. Drug resistance has become a global issue, necessitating the implementation of an integrated parasite management program. A rapid DNA test using loop-mediated isothermal amplification was developed for the detection of F. hepatica from faecal and water samples, enabling real-time decision making to control parasite prevalence.
Fasciola hepatica, commonly referred to as liver flukes, is a substantial zoonotic parasitic disease of humans and livestock globally. While infection is readily controlled by anthelmintics, namely triclabendazole, the heavy reliance on triclabendazole has resulted in drug resistance appearing worldwide. Due to drug resistance, it is imperative to adopt an integrated parasite management program to preserve the efficacy of currently available anthelmintics. A integrated liver fluke management plan would benefit from a simple rapid, field-deployable diagnostic for detection of F. hepatica in environment and the host. Therefore, a rapid DNA test using loop-mediated isothermal amplification was developed and optimised for the detection of F. hepatica from faecal and water samples to enable the detection of parasites both within the host and from the environment. The assay presented here is fast, with amplification in <= 20 min, and highly sensitive, with a detection limit of 5 x 10(-4) ng/mu L. The workflow presented here provides a time to result of <= 60 min without requiring a commercial kit for the extraction of DNA from faecal and water samples, and pending further validation from field-samples, could potentially be used to enable real-time decision making to mitigate parasite prevalence on a farming property and with no requirement for sample transportation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据