Construction of Effective Nanosensor by Combining Semiconducting Polymer Dots with Diphenylcarbazide for Specific Recognition of Trace Cr (VI) Ion in Water and Vitro

Hexavalent chromium (Cr (VI)) ion, as highly toxic environmental pollution, severely endangers the ecological environment and public health. Herein, a fluorescent nanosensor (PFO-DPC) was constructed by combining semiconducting polymer dots with diphenylcarbazide (DPC) for sensing Cr (VI) ion in aqueous solution and living cells. DPC and poly (styrene-co-maleic anhydride) (PSMA) polymer mixed with polyfluorene (PFO) were utilized for selectively indicating Cr (VI) ion and improving the efficiency of detection, respectively. The presence of Cr (VI) ion effectively turned off the blue and green fluorescence of PFO-DPC in the aqueous environment, and the fluorescence quenching efficiency exhibited a good linear relationship between the range of 0.0 to 2.31 nM (R-2 = 0.983) with a limit of detection (LOD) of 0.16 nM. The mechanism of fluorescence quenching could possibly be attributed to the internal filtration effect (IFE). Additionally, PFO-DPC showed a satisfactory performance in monitoring intracellular Cr (VI) ion. Our results indicate that the sensor is promising in various applications.

Automated summary

Hexavalent chromium is a highly toxic environmental pollutant that endangers the ecological environment and public health. This study successfully constructed a fluorescent nanosensor for detecting hexavalent chromium ion in aqueous solution and living cells. The sensor demonstrated selective indication and efficient detection, and showed satisfactory performance in monitoring intracellular hexavalent chromium ion.