期刊
GENES
卷 13, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/genes13071209
关键词
BcTFIIIA; turnip mosaic virus; disease resistance; pak choi
资金
- Natural Science Foundation of Jiangsu Province [BK20200560]
TFIIIA is a zinc-finger transcription factor involved in post-transcriptional regulation during development. BcTFIIIA, isolated from pak choi, was found to interact with TuMV CP and VPg, negatively regulating viral infection.
TFIIIA is a zinc-finger transcription factor that is involved in post-transcriptional regulation during development. Here, the BcTFIIIA gene was isolated from pak choi. Sequence analysis showed that BcTFIIIA encodes 383 amino acids (aa) with an open reading frame (ORF) of 1152 base pairs (bp). We investigated the subcellular location of BcTFIIIA and found the localized protein in the nucleus. BcTFIIIA was suppressed when the pak choi was infected by the turnip mosaic virus (TuMV). The BcTFIIIA mRNA expression level in a resistant variety was higher than that in a sensitive variety, as determined by qRT-PCR analysis. Yeast two hybrid (Y2H) assay and bimolecular fluorescence complementation (BiFC) suggested that BcTFIIIA interacts with TuMV CP and VPg in vivo, respectively, and in vitro. A virus-induced gene silencing (VIGS) experiment showed that the silencing of BcTFIIIA gene expression in pak choi promoted the accumulation of TuMV. These results suggest that BcTFIIIA negatively regulates viral infection through the interaction with TuMV CP and VPg.
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