期刊
FRONTIERS IN PHYSIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2022.920777
关键词
Salmonella; Enteritidis; vaccines; broilers; nanoparticles
类别
资金
- USDA-NIFA [2017-05035]
- USDA-ARS [2017-05035]
- [58-6040-8-034]
This study investigated the efficacy of a killed Salmonella Chitosan nanoparticle (CNP) vaccine on broilers. The results showed that the CNP vaccine significantly enhanced the immune response to Salmonella antigens in poultry, and reduced gut permeability and Salmonella count after infection. The study suggests that the CNP vaccine is a viable alternative to conventional Salmonella poultry vaccines.
There is a critical need for an oral-killed Salmonella vaccine for broilers. Chitosan nanoparticle (CNP) vaccines can be used to deliver Salmonella antigens orally. We investigated the efficacy of a killed Salmonella CNP vaccine on broilers. CNP vaccine was synthesized using Salmonella enterica serovar Enteritidis (S. Enteritidis) outer membrane and flagella proteins. CNP was stable at acidic conditions by releasing 14% of proteins at pH 5.5. At 17 h post-incubation, the cumulative protein release for CNP was 75% at pH 7.4. Two hundred microliters of PBS with chicken red blood cells incubated with 20 mu g/ml CNP released 0% hemoglobin. Three hundred chicks were allocated into 1) Control, 2) Challenge, 3) Vaccine + Challenge. At d1 of age, chicks were spray-vaccinated with PBS or 40 mg CNP. At d7 of age, chicks were orally-vaccinated with PBS or 20 mu g CNP/bird. At d14 of age, birds were orally-challenged with PBS or 1 x 10(7) CFU/bird of S. Enteritidis. The CNP-vaccinated birds had higher antigen-specific IgY/IgA and lymphocyte-proliferation against flagellin (p < 0.05). At 14 days post-infection, CNP-vaccinated birds reversed the loss in gut permeability by 13% (p < 0.05). At 21 days post-infection, the CNP-vaccinated birds decreased S. Enteritidis in the ceca and spleen by 2 Log(10) CFU/g, and in the small intestine by 0.6 Log(10) CFU/g (p < 0.05). We conclude that the CNP vaccine is a viable alternative to conventional Salmonella poultry vaccines.
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