4.7 Review

Harnessing Clinical Trial and Real-World Data Towards an Understanding of Sex Effects on Drug Pharmacokinetics, Pharmacodynamics and Efficacy

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Summary: This study investigated the expression of P-glycoprotein (P-gp) in the intestine of humans and Wistar rats using RT-PCR, Western blot, and LC-MS/MS techniques, revealing similar trends in gender differences and expression levels along the intestinal tract in both species. Strong positive linear correlation was observed between Western blot and LC-MS/MS data in the human small intestine. Conflicting results were shown in rat small intestinal and colonic P-gp expression between the techniques. The use of LC-MS/MS for quantification provided higher confidence in P-gp expression levels without the need for an internal reference protein.

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