4.7 Article

Jian-Pi-Yi-Shen Formula Improves Adenine-Induced Chronic Kidney Disease via Regulating Tryptophan Metabolism and Aryl Hydrocarbon Receptor Signaling

期刊

FRONTIERS IN PHARMACOLOGY
卷 13, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2022.922707

关键词

chronic kidney disease; traditional Chinese medicine; Jian-Pi-Yi-Shen formula; tryptophan metabolism; aryl hydrocarbon receptor

资金

  1. Natural Science Foundation of China [81973602]
  2. Shenzhen Science and Technology Plan Project [JCYJ20190812161001790, JCYJ20210324111210029, JSGG20191129102216637, JCYJ20180302173708520, JCYJ20180507183842516, ZDSYS201606081515458]
  3. Sanming Project of Medicine in Shenzhen [SZZYSM202111002]
  4. Shenzhen Municipal Health Commission [SZLY2018005]
  5. Natural Science Foundation of Guangdong Province [2020A1515011151]

向作者/读者索取更多资源

This study investigated the renoprotective effect and potential mechanism of a traditional Chinese medicine formula, Jian-Pi-Yi-Shen formula (JPYSF), in treating chronic kidney disease (CKD). The results showed that JPYSF treatment improved renal function and pathology, down-regulated fibrotic markers expression, and modulated tryptophan metabolism and AHR activation to protect against CKD.
Traditional Chinese medicine (TCM) is an important complementary and alternative branch of chronic kidney disease (CKD) therapy. Jian-Pi-Yi-Shen formula (JPYSF) is a TCM formula used for treating CKD with good efficacy. However, the underlying mechanisms of JPYSF in treating CKD remain to be elucidated. The purpose of the present study was to investigate the renoprotective effect and potential mechanism of JPYSF in treating CKD. CKD rat model was induced by feeding a diet containing 0.75% w/w adenine for 4 weeks. JPYSF was given by gavage every day, starting from the 3rd week of the adenine-containing diet and continuing for 4 weeks at the dose of 10.89 g/kg. Renal injury was evaluated by serum creatinine (Scr), blood urea nitrogen (BUN), histopathology, and fibrotic markers expression. Serum levels of tryptophan metabolites were detected by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Aryl hydrocarbon receptor (AHR) signaling was tested by Western blot analysis. The results found that JPYSF treatment significantly lowered Scr and BUN levels, improved renal pathological injury, and down-regulated fibrotic markers expression in CKD rats. Furthermore, JPYSF significantly reduced the levels of 10 tryptophan metabolites in the serum of CKD rats and restored the level of tryptophan. Additionally, the kidney expression of AHR signaling was enhanced in CKD rats and was further suppressed in JPYSF treated rats. These results suggested that JPYSF protected against adenine-induced CKD via modulating tryptophan metabolism and AHR activation.

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