期刊
CHEMELECTROCHEM
卷 9, 期 13, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/celc.202200418
关键词
cellobiose dehydrogenase; direct electron transfer; glucose biosensor; mediated electron transfer; osmium redox polymer
资金
- European Union [813006]
- IReL
- Marie Curie Actions (MSCA) [813006] Funding Source: Marie Curie Actions (MSCA)
Cellobiose dehydrogenase (CDH) is a biocatalyst capable of oxidizing cellobiose and related carbohydrates to generate electrical current. A novel engineered CDH has shown improved performance as a bioelectrocatalyst in glucose biosensors.
Cellobiose dehydrogenase (CDH) is capable of oxidizing cellobiose and related carbohydrates and generating electrical current at carbon-based electrodes through direct electron transfer (DET) or mediated electron transfer (MET) mechanisms. As a result, CDHs have been utilized as biocatalysts in biosensors and biofuel cell anodes. A novel engineered ascomycetous Class II CDH with enhanced glucose activity was tested as a bioelectrocatalyst for application to DET or MET-based glucose biosensors with the electrode component amount selection optimized for maximum current in 5 mM glucose solutions. The optimised DET biosensor showed a similar sensitivity and 3-fold lower K-M,K-app when compared to non-optimised DET sensor based on the same engineered CDH. The optimized MET biosensor had a similar K-M,K-app to non-optimized MET biosensor. However, it showed 15-fold improvement in j(max) and 17-fold improvement in sensitivity over the DET biosensor. The sensor signals are not affected by the presence of oxygen, although operation in artificial serum results in 43 % and 28 % lower sensitivity for the DET and MET sensors, respectively. While no individually tested potential interferent breaches a mean absolute relative difference of 20 % of the current, the cumulative co-operative effect in complex media, such as artificial serum, decreases the glucose oxidation current signal.
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