期刊
JOURNAL OF ENDODONTICS
卷 42, 期 3, 页码 460-464出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2015.12.016
关键词
Bacterial reduction; biofilm; disinfection; Enterococcus faecalis; large root canal; passive ultrasonic irrigation
资金
- 90th anniversary of the Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund) [53]
- Special Task force for Activating Research (STAR) under Chulalongkorn University Centenary Fund
Introduction: This study compared the effectiveness of different disinfection protocols in reducing bacteria in an Enterococcus faecalis biofilm in teeth with large root canals. Methods: Fifty-five roots were prepared from human mandibular premolars with large single root canals, and 50 roots were infected with E. faecalis for 21 days. Four roots were observed using scanning electron microscopy to verify biofilm formation. The remaining specimens were assigned into 5 experimental groups and a sterile control group: mechanical instrumentation using files size 6090 and 2.5% sodium hypochlorite (NaOCI) (MI-N), irrigation with 2.5% NaOCI (IR-N), irrigation with 2.5% NaOCI using intermittent passive ultrasonic irrigation (PUI-N), irrigation with normal saline, and no intervention (initial). After root canal disinfection, dentin specimens were collected for microbial analysis. Mean colony forming units were determined and compared between groups using 1-way analysis of variance. Results: The lowest number of intracanal bacteria (24.5 colony-forming units/mL) was recovered from the MI-N group followed by the PUI-N and IR-N groups. The irrigation with normal saline group did not show a significant reduction compared with the initial group. However, there were significant differences between groups (P < .01). The remaining bacteria in the PUI-N group was 4.5-fold lower compared with the IR-N group; however, it was 1862-fold higher compared with the MI-N group. Conclusions: MI-N was the most effective method to disinfect large root canals. Although ultrasonic activation enhanced the efficacy of root canal irrigation, it could not substitute for mechanical instrumentation even in large root canals with unlimited irrigant access to the apical portion.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据