期刊
JOURNAL OF ENDOCRINOLOGY
卷 230, 期 3, 页码 325-337出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/JOE-16-0162
关键词
aquaporin; prolactin; cortisol; gill; osmoregulation
资金
- Skidmore College
- Japan Society for the Promotion of Science [2440164]
- NSF [IOS-1119693]
- Edwin W Pauley Foundation
- National Oceanic and Atmospheric Administration - University of Hawai'i Sea Grant College Program, SOEST [NA14OAR4170071, R/SS-12]
- University of Hawai'i NSF EPSCoR program - Core Genetics Facility at the Hawai'i Institute of Marine Biology [EPS-0903833]
- University of Hawai'i Sea [UNIHI-SEAGRANT-JC-15-02]
- Grants-in-Aid for Scientific Research [26252032, 16K14967, 16H07243] Funding Source: KAKEN
Aquaporins (Aqps) are expressed within key osmoregulatory tissues where they mediate the movement of water and selected solutes across cell membranes. We leveraged the functional plasticity of Mozambique tilapia (Oreochromis mossambicus) gill epithelium to examine how Aqp3, an aquaglyceroporin, is regulated in response to osmoregulatory demands. Particular attention was paid to the actions of critical osmoregulatory hormones, namely, prolactin (Prl), growth hormone and cortisol. Branchial aqp3 mRNA levels were modulated following changes in environmental salinity, with enhanced aqp3 mRNA expression upon transfer from seawater to freshwater (FW). Accordingly, extensive Aqp3 immunoreactivity was localized to cell membranes of branchial epithelium in FW-acclimated animals. Upon transferring hypophysectomized tilapia to FW, we identified that a pituitary factor(s) is required for Aqp3 expression in FW. Replacement with ovine Prl (oPrl) was sufficient to stimulate Aqp3 expression in hypophysectomized animals held in FW, an effect blocked by coinjection with cortisol. Both oPrl and native tilapia Prls (tPrl(177) and tPrl(188)) stimulated aqp3 in incubated gill filaments in a concentration-related manner. Consistent with in vivo responses, coincubation with cortisol blocked oPrl-stimulated aqp3 expression in vitro. Our data indicate that Prl and cortisol act directly upon branchial epithelium to regulate Aqp3 in tilapia. Thus, within the context of the diverse actions of Prl on hydromineral balance in vertebrates, we define a new role for Prl as a regulator of Aqp expression.
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