期刊
FRONTIERS IN MICROBIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.899150
关键词
biofilm; cyanobacteria; transposon library; RNA-Seq; RB-TnSeq
类别
资金
- National Science Foundation
- US-Israel Binational Science Foundation [NSF-BSF 2012823]
- Israel Science Foundation [ISF 1406/14, 2494/19]
In this study, transcriptomics and phenomics screens were used to identify genes involved in biofilm formation and regulation in Synechococcus elongatus PCC 7942. The study also revealed the transcriptomic adaptation to the biofilm state and compared the effectiveness of these two approaches for gene discovery.
Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of small secreted proteins that promotes biofilm formation and a self-suppression mechanism that prevents their expression. Little is known about the regulatory and structural components of the biofilms in PCC 7942, or response to the suppressor signal(s). We performed transcriptomics (RNA-Seq) and phenomics (RB-TnSeq) screens that identified four genes involved in biofilm formation and regulation, more than 25 additional candidates that may impact biofilm formation, and revealed the transcriptomic adaptation to the biofilm state. In so doing, we compared the effectiveness of these two approaches for gene discovery.
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