4.6 Article

Metagenomic and Metabolomic Insights Into the Mechanism Underlying the Disparity in Milk Yield of Holstein Cows

期刊

FRONTIERS IN MICROBIOLOGY
卷 13, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.844968

关键词

Holstein cow; rumen metagenomics; metabolomics; milk yield; citric acid cycle

资金

  1. National Key R&D Program of China [2021YFF1000703-01]
  2. 333 Talent Plan Program of Jiangsu Province, China
  3. High-Performance Computing Platform of Bioinformatics Center, Nanjing Agricultural University

向作者/读者索取更多资源

This study investigates the metabolic mechanism underlying the difference in milk yield of Holstein cows. The findings reveal differences in microbiota and metabolites between high milk yield and low milk yield groups. The study suggests that upregulated dopamine levels in the rumen fluid and milk may contribute to reduced milk yield, while upregulated citrulline and N-acetylornithine potentially enhance energy metabolism and gluconeogenesis, leading to high milk yield.
This study was conducted to investigate the metabolic mechanism underlying the disparity in the milk yield of Holstein cows. Eighteen lactating Holstein cows in their second parity and 56 (+/- 14.81 SD) days in milking (DIM) were selected from 94 cows. Based on the milk yield of the cows, they were divided into two groups of nine cows each, the high milk yield group (HP) (44.57 +/- 2.11 kg/day) and the low milk yield group (LP) (26.71 +/- 0.70 kg/day). The experimental cows were fed the same diet and kept under the same management system for more than 60 days. Rumen metagenomics revealed that two Archaea genera, one Bacteria genus, eight Eukaryota genera, and two Virus genera differ between the HP and LP groups. The analysis of metabolites in the rumen fluid, milk, and serum showed that several metabolites differed between the HP and LP groups. Correlation analysis between the predominant microbiota and milk yield-associated metabolites (MP-metabolites) revealed that four Bacteria and two Eukaryota genera have a positive relationship with MP-metabolites. Pathway enrichment analysis of the differential metabolites revealed that five pathways were enriched in all the samples (two pathways in the milk, two pathways in the serum, and one pathway in the rumen fluid). Further investigation revealed that the low milk yield observed in the LP group might be due to an upregulation in dopamine levels in the rumen fluid and milk, which could inhibit the release of prolactin or suppress the action of oxytocin in the udder resulting in reduced milk yield. On the other hand, the high milk yield in the HP group is attributed to an upregulation in citrulline, and N-acetylornithine, which could be used as substrates for energy metabolism in the citric acid cycle and ultimately gluconeogenesis.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据