期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2022.942457
关键词
S. aureus; S. epidermidis; S. mitis; septic arthritis; mice
资金
- Swedish Medical Research Council [523-2013-2750, 2019-01135]
- Swedish Government
- county councils
- ALF-agreement [ALFGBG-823941]
- Rune och Ulla Amlovs Stiftelse for Neurologisk och Reumatologisk Forskning [2016-075]
- E och K.G. Lennanders stipendiestiftelse
- Magnus Bergvalls Stiftelse [2017-01958, 2018-02797]
- Sahlgrenska University Hospital Foundations
- Institute of Medicine, Gothenburg University
- Swedish Research Council [2019-01135] Funding Source: Swedish Research Council
This study found that human commensal bacteria can augment Staphylococcus aureus-induced septic arthritis. This augmentation effect is dose-dependent and can lead to worsened clinical symptoms and pathological changes.
Background: Septic arthritis is considered one of the most dangerous joints diseases and is mainly caused by the Gram-positive bacterium Staphylococcus aureus (S. aureus). Human skin commensals are known to augment S. aureus infections. The aim of this study was to investigate if human commensals could augment S. aureus-induced septic arthritis. Method: NMRI mice were inoculated with S. aureus alone or with a mixture of S. aureus together with either of the human commensal Staphylococcus epidermidis (S. epidermidis) or Streptococcus mitis (S. mitis). The clinical, radiological and histopathological changes due to septic arthritis were observed. Furthermore, the serum levels of chemokines and cytokines were assessed. Results: Mice inoculated with a mixture of S. aureus and S. epidermidis or S. mitis developed more severe and frequent clinical arthritis compared to mice inoculated with S. aureus alone. This finding was verified pathologically and radiologically. Furthermore, the ability of mice to clear invading bacteria in the joints but not in kidneys was hampered by the bacterial mixture compared to S. aureus alone. Serum levels of monocyte chemoattractant protein 1 were elevated at the early phase of disease in the mice infected with bacterial mixture compared with ones infected with S. aureus alone. Finally, the augmentation effect in septic arthritis development by S. epidermidis was bacterial dose-dependent. Conclusion: The commensal bacteria dose-dependently augment S. aureus-induced septic arthritis in a mouse model of septic arthritis.
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