期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2022.871135
关键词
Mycobacterium tuberculosis; cyclic-di-AMP; phosphodiesterase; immune response; infection; vaccine
资金
- National Major Special Projects of the 13th Five-year Plan [2018ZX10302302002004]
- National Natural Science Foundation [81971560, 81671638, 81371774]
- Provincial Natural Science Foundation of Shaanxi Province [2022ZDLSF01-07]
Many antigens from Mycobacterium tuberculosis have strong immunogenicity and potential as vaccine candidate antigens. In this study, the researchers explored the immunogenicity and immune responses induced by the bacterial second messenger Cyclic di-AMP (c-di-AMP) phosphodiesterase (CnpB) in mice. They found that CnpB had strong immunogenicity and induced high levels of humoral response and lung mucosal immunity after M. tuberculosis infection. CnpB also interfered with host innate and adaptive immune responses and conferred protection against M. tuberculosis respiratory infection.
Many antigens from Mycobacterium tuberculosis (M. tuberculosis) have been demonstrated as strong immunogens and proved to have application potential as vaccine candidate antigens. Cyclic di-AMP (c-di-AMP) as a bacterial second messenger regulates various bacterial processes as well as the host immune responses. Rv2837c, the c-di-AMP phosphodiesterase (CnpB), was found to be relative to virulence of M. tuberculosis and interference with host innate immune response. In this study, recombinant CnpB was administered subcutaneously to mice. We found that CnpB had strong immunogenicity and induced high levels of humoral response and lung mucosal immunity after M. tuberculosis intranasally infection. CnpB immunization stimulated splenocyte proliferation and the increasing number of activated NK cells but had little effects on Th1/Th2 cellular immune responses in spleens. However, CnpB induced significant Th1/Th2 cellular immune responses with a decreased number of T and B cells in the lungs, and significantly recruits of CD4(+) and CD8(+) T cells after M. tuberculosis attenuated strain H37Ra infection. Besides, we first reported that CnpB could stimulate IFN-beta expression transitorily and inhibit the autophagy of macrophages in vitro. In mice intranasally infection model, CnpB immunization alleviated pathological changes and reduced M. tuberculosis H37Ra loads in the lungs. Thus, our results suggested that CnpB interferes with host innate and adaptive immune responses and confers protection against M. tuberculosis respiratory infection, which should be considered in vaccine development as well as a drug target.
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