期刊
ELIFE
卷 11, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.74327
关键词
circadian; sleep homeostasis; ellipsoid body; Drosophila; clock; D; melanogaster
类别
资金
- National Institutes of Health [R01NS106955]
- Army Research Office [W911NF1610584]
- National Science Foundation [DMS-1764421]
- Simons Foundation [597491-RWC]
- Northwestern University
- National Institute of Neurological Disorders and Stroke [F32 NS110183]
- U.S. Department of Defense (DOD) [W911NF1610584] Funding Source: U.S. Department of Defense (DOD)
This study reveals the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center in fruit flies. The effects of sleep deprivation depend on morning and evening clock neurons, independent of their roles in circadian locomotor activity. Higher morning rebound and elevated gene and protein expression levels as well as calcium levels in sleep homeostat neurons suggest regulation by clock circuits.
Homeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects depend on discrete morning and evening clock neurons, independent of their roles in circadian locomotor activity. In the R5 ellipsoid body sleep homeostat, we identified elevated morning expression of activity dependent and presynaptic gene expression as well as the presynaptic protein BRUCHPILOT consistent with regulation by clock circuits. These neurons also display elevated calcium levels in response to sleep loss in the morning, but not the evening consistent with the observed time-dependent sleep rebound. These studies reveal the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center.
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