期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 10, 期 28, 页码 9041-9052出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.2c00948
关键词
switchgrass; organosolv pretreatment; lignin structure; transgenic switchgrass; small-angle X-ray scattering
资金
- Genomic Science Program [FWP ERKP752]
- U.S. Department of Energy (DOE) O ffi ce of Science User Facility [DE-SC0012704]
- DOE O ffi ce of Science by Brookhaven National Laboratory [P30GM133893]
- Center for BioMolecular Structure (CBMS) [KP1605010]
- National Institutes of Health [DE-AC05-00OR22725]
- National Institute of General Medical Sciences (NIGMS) through a Center Core P30 Grant
- DOE O ffi ce of Biological and Environmental Research
- INCITE award
- O ffi ce of Science of the U.S. Department of Energy
- U.S. Department of Energy
- United States Government
The study revealed significant structural changes in solubilized lignin isolated from wild-type and transgenic switchgrass after different organosolv pretreatments, with ethanol pretreatment causing the most pronounced reduction in lignin molecular mass. THF-pretreated transgenic lignins showed higher molecular mass, more fi-O-4 linkages, and aliphatic hydroxyl content compared to ethanol and GVL pretreated lignin.
The recalcitrance of lignocellulosic biomass is a challenge in biological-based biorefinery systems due to the complex physicochemical structure of plant cell walls. Pretreatment and genetic modification are two approaches in biomass conversion that have succeeded in modifying the structure of lignocellulose to enable better enzymatic deconstruction. However, the structural differences among pretreatment-solubilized lignin isolated from switchgrass genotypes have not been extensively investigated. Here, three organosolv pretreatment systems -ethanol (EtOH), tetrahydrofuran (THF), and y-valerolactone (GVL)-were used on wild type (WT) and two transgenic switchgrasses. All organosolv pretreatments caused a significant reduction in the molecular mass of lignins; particularly, up to & SIM;90% decrease was observed in EtOH-pretreated lignin compared to untreated lignin. The WT EtOH lignin also presented the smallest particle size among all WT lignins. THF pretreated transgenic lignins showed a higher molecular mass, fi-O-4 linkages, and aliphatic hydroxyl content compared to EtOH and GVL pretreated lignin. The number of hydrogen bonds between lignin and the organic solvents calculated from the molecular dynamics simulations followed the same trend as the experimentally determined reduction in lignin molecular mass. The results revealed the structural changes of solubilized lignin isolated from wild-type and transgenic switchgrass after different organosolv pretreatments.
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