期刊
TOXINS
卷 14, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/toxins14070438
关键词
lung cancer; antimicrobial peptide; scorpion; venom; tumor
资金
- Chinese National Natural Science Foundation [31861143050, 31772476, 31911530077, 82070038]
- Academy of Scientific Research and Technology (ASRT, Egypt)
- (China-Egypt Scientific and Technological Cooperation Program)
Smp24, a cationic antimicrobial peptide from the venom gland of the Egyptian scorpion Scorpio maurus palmatus, has been found to suppress the growth of lung cancer cells and show low toxicity to normal cells. It induces necrosis of lung cancer cells by disrupting the integrity of cell membranes and cytoskeleton, and also inhibits cell motility. Additionally, Smp24 demonstrates effective anticancer protection in a mouse model and low acute toxicity.
Smp24, a cationic antimicrobial peptide identified from the venom gland of the Egyptian scorpion Scorpio maurus palmatus, shows variable cytotoxicity on various tumor (KG1a, CCRF-CEM and HepG2) and non-tumor (CD34(+), HRECs, HACAT) cell lines. However, the effects of Smp24 and its mode of action on lung cancer cell lines remain unknown. Herein, the effect of Smp24 on the viability, membrane disruption, cytoskeleton, migration and invasion, and MMP-2/-9 and TIMP-1/-2 expression of human lung cancer cells have been evaluated. In addition, its in vivo antitumor role and acute toxicity were also assessed. In our study, Smp24 was found to suppress the growth of A549, H3122, PC-9, and H460 with IC50 values from about 4.06 to 7.07 mu M and show low toxicity to normal cells (MRC-5) with 14.68 mu M of IC50. Furthermore, Smp24 could induce necrosis of A549 cells via destroying the integrity of the cell membrane and mitochondrial and nuclear membranes. Additionally, Smp24 suppressed cell motility by damaging the cytoskeleton and altering MMP-2/-9 and TIMP-1/-2 expression. Finally, Smp24 showed effective anticancer protection in a A549 xenograft mice model and low acute toxicity. Overall, these findings indicate that Smp24 significantly exerts an antitumor effect due to its induction of membrane defects and cytoskeleton disruption. Accordingly, our findings will open an avenue for developing scorpion venom peptides into chemotherapeutic agents targeting lung cancer cells.
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